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Product Info Summary
| SKU: | M00020-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CTLA4 Monoclonal Antibody [1E6]
SKU/Catalog Number
M00020-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CTLA4 Monoclonal Antibody [1E6] (Catalog # M00020-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CTLA4 Monoclonal Antibody [1E6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00020-1)
Host
Mouse
Contents
CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 1E6
Isotype
IgG1
Immunogen
CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
Reactive Species
M00020-1 is reactive to CTLA4 in Human
Applications
M00020-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.656kDa
Background of CTLA-4
The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 μg/mL.
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Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
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Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL.
Red: CTLA4 Antibody [1E6] (RF16011)
Blue: DAPI staining
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Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL.
Red: CTLA4 Antibody [1E6] (RF16011)
Blue: DAPI staining
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Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL.
Red: CTLA4 Antibody [1E6] (M00020-1)
Blue: DAPI staining
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Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.
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Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CTLA4 (Source: Uniprot.org, NCBI)
Gene Name
CTLA4
Full Name
Cytotoxic T-lymphocyte protein 4
Weight
24.656kDa
Alternative Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 CTLA4 ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 cytotoxic T-lymphocyte associated protein 4 cytotoxic T-lymphocyte protein 4|celiac disease 3|cytotoxic T lymphocyte associated antigen 4 short spliced form|cytotoxic T-lymphocyte-associated serine esterase-4|insulin-dependent diabetes mellitus 12|ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTLA4, check out the CTLA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTLA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTLA4 Monoclonal Antibody [1E6] (M00020-1)
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No publications found for M00020-1
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